Effect of cycloheximide on the cell cycle of the crypts of the small intestine of the rat.
J Cell Biol
; 35(3): 649-58, 1967 Dec.
Article
em En
| MEDLINE
| ID: mdl-6064368
ABSTRACT
A single injection of 1.5 mg/kg of cycloheximide induces a complete disappearance of mitotic activity in rat intestinal crypts within 1.5-2 hr. No significant necrosis of crypt cells is observed even though this phenomenon is accompanied by a marked decrease in uptake of labeled precursors into protein and DNA. Mitoses reappear 6 hr after injection and recovery then follows a cyclic pattern over a period equivalent to one cell cycle, thereby reflecting at least a partial synchronization of cell division. Concurrent use of colchicine, an agent known to induce metaphase arrest, has demonstrated that cycloheximide, while having no apparent effect on cells already in division, prevents the entrance of new cells into visible mitosis. Analysis of the cell cycle suggests that one block initiated by cycloheximide occurs in G(2), presumably as the result of an interference with the formation of protein(s) required for the normal progression of cells from this phase of the cycle into mitosis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Cicloeximida
/
Mitose
Limite:
Animals
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
1967
Tipo de documento:
Article