Transformation of LMTK- cells with purified HLA class I gene. VI. Serological characterization of HLA-B7 and AW24 molecules.
Hum Immunol
; 11(1): 31-45, 1984 Sep.
Article
em En
| MEDLINE
| ID: mdl-6207154
ABSTRACT
Serological characterization of HLA-B7 and HLA-AW24 class I molecules following transfection of murine LMTK- cells with purified HLA class I genes was performed using human alloantisera. Induction by murine alpha interferon of the expression of class I molecules was required to obtain unambiguous identification of these molecules which appear serologically identical to the HLA-B7 and HLA-AW24 molecules expressed at the surface of human peripheral blood lymphocytes of 20 unrelated individuals. Analysis of the transformed cells with 8 different anti-HLA class I monoclonal antibodies results in the definition of 3 separate clusters of antigenic determinants shared by all HLA class I molecules. These studies further suggest the existence of locus-specific serological reactivities associated either with the HLA-A or with the HLA-B and C gene products.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Timidina Quinase
/
Transfecção
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Antígenos HLA-A
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Genes MHC da Classe II
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Genes
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Antígenos HLA
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Complexo Principal de Histocompatibilidade
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Hum Immunol
Ano de publicação:
1984
Tipo de documento:
Article