Photoaffinity labeling of alpha- and beta- scorpion toxin receptors associated with rat brain sodium channel.
Biochem Biophys Res Commun
; 115(2): 415-22, 1983 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-6312978
ABSTRACT
Azido nitrophenylaminoacetyl [125I]iodo derivative of toxin II from Centruroides suffusus suffusus, a beta-toxin, and azido nitrophenylaminoacetyl [125I]iodo derivative of toxin V from Leiurus quinquestriatus quinquestriatus, an alpha-toxin, have been covalently linked after binding to their receptor sites that are related to the voltage sensitive sodium channel present in rat brain synaptosomes. Both derivatives labeled two polypeptides of 253000 +/- 20000 and 35000 +/- 2000 mol. wt. Labeling was blocked for each derivative by a large excess of the corresponding native toxin but no cross inhibition was obtained. These results suggest that both alpha - and beta - scorpion toxin receptors are located on or near the same two membrane polypeptides which may be part of the voltage dependent sodium channel.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sódio
/
Encéfalo
/
Canais de Sódio
/
Receptores Colinérgicos
/
Canais Iônicos
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1983
Tipo de documento:
Article