Radioimmunoassay of neocarzinostatin, an antitumor protein.
Cancer Res
; 36(12): 4378-81, 1976 Dec.
Article
em En
| MEDLINE
| ID: mdl-63321
ABSTRACT
Antibodies directed toward the antitumor protein neocarzinostatin (NCS) have been produced in a rabbit by immunization with a highly purified NCS preparation. The antiserum was monospecific and reversed the antibacterial activity of NCS against Sarcina lutea. It cross-reacted with chemically modified derivatives of NCS and mitomalcin but failed to cross-react with macromomycin. A radioimmunoassay procedure has been developed utilizing the antiserum and a biologically active 125I-labeled derivative of NCS. The lower limit of detection by this radioimmunoassay, which involves a double antibody technique for the separation of antibody-bound and free antigen, was 1 X 10(-13) mole. The sensitivity of the assay is such that serum levels of NCS can be determined accurately after administration of the drug to rats at a single dose of 2 mg/kg. Since NCS is now undergoing clinical trial, the radioimmunoassay of the drug will be a valuable tool in clinical pharmacological studies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Radioimunoensaio
/
Zinostatina
/
Antibióticos Antineoplásicos
Limite:
Animals
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1976
Tipo de documento:
Article