Synthesis of yeast histone 3 in an Escherichia coli cell-free system.
J Mol Biol
; 168(3): 489-503, 1983 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-6350605
ABSTRACT
The gene for histone H3 from the yeast Saccharomyces cerevisiae was placed under the control of the lac promoter of Escherichia coli by fusing the H3 coding sequence to that of beta-galactosidase. The gene was shown to be transcribed in vivo, but its product was not detected in cell extracts. However, synthesis of the fused polypeptide was detected in an in vitro transcription-translation system derived from E. coli. Proteolytic degradation of the newly synthesized polypeptides may be the cause of their apparent absence in the in vivo experiment.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Histonas
/
Escherichia coli
Idioma:
En
Revista:
J Mol Biol
Ano de publicação:
1983
Tipo de documento:
Article