Prostacyclin (PGI2) synthesis in the vascular wall of rats with bilateral renal artery stenosis.
Hypertension
; 5(6 Pt 3): V38-42, 1983.
Article
em En
| MEDLINE
| ID: mdl-6360881
ABSTRACT
The ability of vessels (rings of arteries and vena cava) to synthesize prostacyclin (PGI2) "in vitro" was analyzed in the initial (6-day) and chronic (6-week) phase of two-kidney, two clip hypertension. Male Wistar CHbb THOM rats were used. Tissues were incubated for two hours in Krebs solution containing 14C-arachidonic acid as exogenous substrate. Specimens (in benzene-ethanol 41 vol/vol) and the unlabeled standard solutions of arachidonic acid, 6-keto PGF1 alpha, PGF2 alpha, and PGE2, were spotted on silica gel-G plates for thin layer chromatography. Conversion of 14C-arachidonic acid to stable metabolite 6-keto PGF1 alpha was used as an index of PGI2 synthesis. Results shown 1) PGI2 is the major PG synthesized by the rat artery wall; 2) PGI2 synthesis was increased 2.4 times in the initial 6-day period of development of renovascular hypertension (RH); 3) no changes in PGI2 production were observed in arteries during the chronic 6-week period of RH; 4) abdominal vena cava has little or no capacity to produce PGI2. As PGI2 is a potent vasodilator, higher production by arteries during the 6-day period suggests that prostacyclin could play a modulator role on peripheral resistance during the initial phase of renal hypertension.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Obstrução da Artéria Renal
/
Vasos Sanguíneos
/
Epoprostenol
/
Hipertensão Renovascular
Limite:
Animals
Idioma:
En
Revista:
Hypertension
Ano de publicação:
1983
Tipo de documento:
Article