Molecular species of monoglycosylceramides in embryonic and adult Japanese quail intestine.
Biochim Biophys Acta
; 796(3): 269-76, 1984 Dec 06.
Article
em En
| MEDLINE
| ID: mdl-6509078
ABSTRACT
Molecular species of monoglycosylceramides from embryonic and adult Japanese quail intestine were separated without derivatization by high performance liquid chromatography and analyzed by fast atom bombardment-mass spectrometry. The reversed-phase chromatography on an octadecyl silica beads column separated the lipids by the difference in their ceramide moieties which contain considerable amounts of hydroxy fatty acids and 4D-hydroxysphinganine (phytosphingosine). The phytosphingosine and sphingosine in the ceramide moieties of the isolated monoglycosylceramide fraction gave characteristic fragment ions of (M - 162)H+ for the phytosphingosine, and (M - 18)H+ for the sphingosine components. The presence of phytosphingosine and hydroxy fatty acids in the ceramide moieties of the monoglycosylceramide early in the embryonic stage necessarily excludes the possibility of their exogenous origin such as bacterial flora and nutrients.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cerebrosídeos
/
Intestinos
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1984
Tipo de documento:
Article