An Ecteola-cellulose chromatography assay for 3'-phosphoadenosine 5'-phosphosulfate: phenol sulfotransferase.
Anal Biochem
; 130(2): 334-8, 1983 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-6575625
ABSTRACT
A new assay procedure for phenol sulfotransferase which employs [35S]-3'-phosphoadenosine-5'-phosphosulfate as a sulfate donor and a variety of phenols as sulfate acceptors was developed. The appearance of the 35S-sulfated products or the disappearance of the [35S]-3'-phosphoadenosine-5'-phosphosulfate are determined simultaneously by chromatography of the assay incubation mixtures on Ecteola-cellulose columns, eluting with an NH4HCO3 step gradient. Various acidic, neutral, and basic phenols can be employed as substrates for phenol sulfotransferase using this procedure.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sulfurtransferases
/
Sulfotransferases
Limite:
Animals
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
1983
Tipo de documento:
Article