Antibody elution from red blood cells by chloroform and trichloroethylene.
Transfusion
; 22(5): 359-61, 1982.
Article
em En
| MEDLINE
| ID: mdl-6750874
ABSTRACT
Antibody elution by chloroform and trichloroethylene was compared with the Rubin ether method modified by Hughes-Jones for IgG antibodies and the Landsteiner and Miller heat method for IgM antibodies. After washing the cells, the chloroform and trichloroethylene method was done at 37 degrees C for 10 minutes. With this technique the eluate remained on the upper layer above the red blood cell stroma layer, while with Rubin's method the eluate remained below the cell stroma. The reported technique was as effective as the Landsteiner and Miller heat method for IgM antibodies, and a double volume of eluate was obtained.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antígenos de Grupos Sanguíneos
/
Clorofórmio
/
Técnicas Imunológicas
/
Isoanticorpos
Limite:
Humans
/
Newborn
Idioma:
En
Revista:
Transfusion
Ano de publicação:
1982
Tipo de documento:
Article