An observation chamber for a high-pressure stopped-flow apparatus.
J Biochem Biophys Methods
; 6(2): 173-7, 1982 Jun.
Article
em En
| MEDLINE
| ID: mdl-6809803
ABSTRACT
A new design of the observation window to observation chamber seal is described for the Berger rapid stopped-flow apparatus. This design permits reliable operation of the apparatus and retains the features of a square non-deviating observation path with flush windows. Incorporation of fiber optic light guides for the observation path has made the apparatus insensitive to vibration artifacts. A demonstration reaction of calcium binding to EGTA is presented; the reaction, which is 90% complete within 2 ms, illustrates the capability of this apparatus for examining fast reactions.
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Base de dados:
MEDLINE
Assunto principal:
Bioquímica
Idioma:
En
Revista:
J Biochem Biophys Methods
Ano de publicação:
1982
Tipo de documento:
Article