Effector--target cell interaction of lymph node cells from galactocerebroside-sensitized rats with oligodendrocytes of brain cell cultures.

ABSTRACT

Inbred Lewis rats were sensitized with galactocerebrosides (GC) and hemocyanin. After 10 years lymph node cells (LNC) depleted of phagocytic cells were obtained. These cells were tested on mixed brain cell cultures enriched with GC-positive oligodendrocytes. Within 3--48 h of incubation, conjugate formation between LNC and oligodendrocytes was observed. Interaction of effector cells with their target resulted in defective oligodendrocytes. Anti-GC-LNC did not adhere to or lyse astrocytes or fibroblasts. Control LNC from rats sensitized only with the carrier protein did not interact with oligodendrocytes.