A simple device for injection of small calibrated amounts of horseradish peroxidase into the cerebral cortex.
J Neurosci Methods
; 2(1): 47-9, 1980 Feb.
Article
em En
| MEDLINE
| ID: mdl-7035757
ABSTRACT
A simple device for injecting horseradish peroxidase into the cerebral cortex is described. It consists of a syringe connected by tubing to a hydraulic adaptor which provides a sealed connection to a disposable glass micropipette. Replaceable micropipettes may be prepared by drawing capillary glass under heat to a fine diameter tip that provides easy penetration into the cortex without dimpling the surface grey matter or damaging the underlying white matter. The hydraulic seal achieved by the adaptor allows volumes of the order of 0.05 microliter to be readily injected into nervous tissue.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Córtex Cerebral
/
Técnicas Histológicas
Limite:
Animals
Idioma:
En
Revista:
J Neurosci Methods
Ano de publicação:
1980
Tipo de documento:
Article