In vitro evaluation of hematopoiesis in mice treated with busulphan or nitrogen mustard.
Biomed Pharmacother
; 36(3): 149-52, 1982.
Article
em En
| MEDLINE
| ID: mdl-7150708
ABSTRACT
Long term bone marrow cultures were established using combinations of untreated marrow and marrow treated with nitrogen mustard or busulphan in vivo. This study showed that 1) adherent cell layers, which morphologically resemble those derived from normal marrow, can be established from mice treated with busulphan or nitrogen mustard, 2) total cellularity in cultures containing a treated marrow is drastically reduced, 3) CFU-S content was slightly below control values in cultures of treated non-adherent cells on normal monolayers, and 4) CFU-S content was severely reduced when the monolayer was derived from drug-treated marrow. The usefulness of this long term culture system to study drug-induced modulation of hematopoiesis has been substantiated by this study.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bussulfano
/
Hematopoese
/
Mecloretamina
Limite:
Animals
Idioma:
En
Revista:
Biomed Pharmacother
Ano de publicação:
1982
Tipo de documento:
Article