Discrimination of Mycobacterium avium-Mycobacterium intracellulare strains by genomic DNA fingerprinting with a 16S rRNA gene probe.
FEMS Microbiol Lett
; 124(1): 75-9, 1994 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-7528153
ABSTRACT
Ribotyping was investigated as a means of distinguishing ten different serotyped reference strains and seven epidemiologically unrelated isolates of Mycobacterium avium-Mycobacterium intracellulare using a labelled 16S rDNA probe. Thirteen restriction enzymes were screened towards an accurate discrimination of strains. Two selected restriction enzymes (SacI and ClaI) enabled us to classify the 17 strains into ten ribotypes with an index of discrimination of 0.897. Typeability and reproducibility of the method reached 100%. The patterns obtained exhibited polymorphism of RE fragments within and outside the 16S rRNA gene and may be useful for epidemiological studies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Ribossômico 16S
/
Sondas de DNA
/
Complexo Mycobacterium avium
/
Impressões Digitais de DNA
/
Mycobacterium avium
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
França