Changes in lipoxygenase activities in human erythroleukemia (HEL) cells during diosgenin-induced differentiation.
Cancer Lett
; 96(1): 133-40, 1995 Sep 04.
Article
em En
| MEDLINE
| ID: mdl-7553601
ABSTRACT
A human erythroleukemia (HEL) cell line was used as a model to study dynamic changes in human 12-, 15-, 5-lipoxygenases, five lipoxygenase activating protein (FLAP), and leukotriene A4 (LTA4) hydrolase gene expression during megakaryocytic differentiation induced by diosgenin (Beneytout, J.L., Nappez, C., Leboutet, M.J. and Malinvaud, G., Biochem. Biophys. Res. Commun., 207 (1995) 398-404). The study was performed at the transcriptional level 12- and 5-lipoxygenase mRNAs, FLAP mRNA and LTA4 hydrolase mRNA were detected before and after diosgenin treatment in HEL cells while 15-lipoxygenase mRNA was undetected. When HEL cells were incubated with arachidonic acid, 5-, 12-, 15-hydroxyeicosatetraenoic acids (HETE) and LTC4 were synthesized. In contrast, the diosgenin treatment induced the suppression of 12-lipoxygenase activity and only 5-, 15-HETEs and LTC4 were synthesized.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Megacariócitos
/
Leucemia Eritroblástica Aguda
/
Diferenciação Celular
/
Lipoxigenase
/
Diosgenina
Limite:
Humans
Idioma:
En
Revista:
Cancer Lett
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
França