Direct agglutination of weak D red cells by tetramolecular complexes containing monoclonal IgG anti-D.
Vox Sang
; 65(2): 141-5, 1993.
Article
em En
| MEDLINE
| ID: mdl-7692674
ABSTRACT
The capacity of blood group antibodies to agglutinate red cells suspended in saline is largely dependent on the antibody isotype. The immunological cross-linking of IgG antibodies has previously been described as a means to increase the reactivity of IgG in many situations. We have prepared anti-D-containing complexes by blending a human IgG anti-D monoclonal antibody (mAb) and a murine anti-human IgG mAb. In standard red cell serology assays, the anti-D complexes exhibited a very high avidity and could agglutinate weak D-positive red cells in direct saline testing. These results indicate that potent saline hem-agglutinating reagents of RhD and eventually of other blood group specificities can be prepared from IgG mAbs.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Sistema do Grupo Sanguíneo Rh-Hr
/
Imunoglobulina G
/
Hemaglutinação
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Vox Sang
Ano de publicação:
1993
Tipo de documento:
Article