Immunocytochemical detection of phosphatidylinositol 4,5-bisphosphate in Epon embedded specimens.
J Submicrosc Cytol Pathol
; 27(1): 115-8, 1995 Jan.
Article
em En
| MEDLINE
| ID: mdl-7697614
ABSTRACT
A comparison between the time-consuming freeze-substitution Lowicryl HM 20 embedding procedure with the Epon embedding method for the immunocytochemical detection of phosphatidylinositol 4,5-bisphosphate (PIP2) by means of a monoclonal antibody is described. The results indicate that the intracellular localization of the phospholipid and the efficiency of its identification are similar, opening the possibility of performing the immunoreaction also on samples that are routinely processed for pathological investigations.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfotransferases (Aceptor do Grupo Álcool)
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Submicrosc Cytol Pathol
Assunto da revista:
PATOLOGIA
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Itália