Serotype-specific detection of bean common mosaic potyvirus in bean leaf and seed tissue by enzymatic amplification.
J Virol Methods
; 50(1-3): 145-54, 1994 Dec.
Article
em En
| MEDLINE
| ID: mdl-7714037
ABSTRACT
An assay involving reverse transcription and polymerase chain reaction (PCR) is described for specific detection of serotypes A and B BCMV isolates in bean leaf and seed tissues. Three oligonucleotide primers designed according to the sequence data available allow, in appropriate combination, serotype-specific detection of BCMV. The sensitivity of the method was sufficient to detect BCMV in as little as 100 fg and 50 pg of infected leaf and seed tissues, respectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas Medicinais
/
Reação em Cadeia da Polimerase
/
Fabaceae
/
Vírus do Mosaico
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Espanha