Tissue factor activity of syncytiotrophoblast plasma membranes and tumoral trophoblast cells in culture.
Thromb Haemost
; 73(1): 49-54, 1995 Jan.
Article
em En
| MEDLINE
| ID: mdl-7740495
ABSTRACT
During pregnancy, important modifications of hemostasis occur resulting in mothers in hypercoagulability and the role of placental cells such as trophoblast cells has been hypothesized. In this study, we first showed that syncytiotrophoblast plasma membranes, isolated from normal human placenta, expressed a strong tissue factor (TF) activity. We then studied TF activity of two continuous trophoblast cell lines (JEG-3 and BeWo) in comparison to human umbilical vein endothelial cells (HUVEC) and transformed human endothelial cells (ECV-304). TF assays were performed on intact detached confluent cells. Unstimulated JEG-3 and BeWo cells exhibited a very high TF activity which slightly increased after 2 to 4 h TNF-alpha stimulation. In contrast, HUVEC and ECV-304 had a lower basal TF activity which was mainly inducible by TNF-alpha, with a maximum effect after 4 to 6 h stimulation. For both cell types, TF activity was decreased to basal value after 16-hour TNF-alpha stimulation. These results support that trophoblast cells are able to express TF but the involvement of this property in the hemostatic physiological changes observed during pregnancy, remains to be demonstrated.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trofoblastos
/
Neoplasias Uterinas
/
Gravidez
/
Tromboplastina
/
Coriocarcinoma
/
Membrana Celular
/
Proteínas de Neoplasias
Limite:
Female
/
Humans
Idioma:
En
Revista:
Thromb Haemost
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
França