Effects of phleomycin-induced DNA damage on the fission yeast Schizosaccharomyces pombe cell cycle.
Yeast
; 11(3): 225-31, 1995 Mar.
Article
em En
| MEDLINE
| ID: mdl-7785323
ABSTRACT
The effect of phleomycin, a bleomycin-like antibiotic, has been investigated in the fission yeast, Schizosaccharomyces pombe. We report that in response to phleomycin-induced DNA damage, growth was inhibited and S. pombe cells arrested in the G2-phase of the cell cycle. DNA repair mutants rad9 and rad17 did not arrest and were hypersensitive to phleomycin. Cell cycle mutants that entered mitosis without monitoring the completion of DNA replication also displayed an increased sensitivity to this DNA-damaging agent. Thus, phleomycin could be used as a tool in the fission yeast S. pombe model system for the study of DNA damage and cell cycle checkpoints, or as a new selective agent.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fleomicinas
/
Schizosaccharomyces
/
Dano ao DNA
Idioma:
En
Revista:
Yeast
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
França