RT-PCR diagnosis of patients with acute nonlymphocytic leukemia and inv(16)(p13q22) and identification of new alternative splicing in CBFB-MYH11 transcripts.
Blood
; 86(1): 277-82, 1995 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-7795233
ABSTRACT
As acute nonlymphocytic leukemia (ANLL) with inv(16) (p13q22) or t(16;16)(p13;q22) has been shown to result from the fusion of transcription factor subunit core binding factor (CBFB) to a myosin heavy chain (MYH11), we sought to design methods to detect this rearrangement using reverse transcriptase-polymerase chain reaction (RT-PCR). In all of 27 inv(16)(p13q22) and four t(16;16)(p13;q22) cases tested, a chimeric CBFB-MYH11 transcript coding for an in-frame fusion protein was detected. In a more extensive RT-PCR analysis with different primer pairs, we detected a second new chimeric CBFB-MYH11 transcript in 10 of 11 patients tested. The CBFB-MYH11 reading frame of the second transcript was maintained in one patient but not in the others. We show that the different CBFB-MYH11 transcripts in one patient arise from alternative splicing. Translation of the transcript in which the CBFB-MYH11 reading frame is not maintained leads to a slightly truncated CBFB protein.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Cromossomos Humanos Par 16
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Leucemia Mieloide Aguda
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Regulação Leucêmica da Expressão Gênica
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Proteínas de Fusão Oncogênica
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Splicing de RNA
/
Reação em Cadeia da Polimerase
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Miosinas
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Proteínas de Ligação a DNA
/
Inversão Cromossômica
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Adolescent
/
Adult
/
Aged
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Child
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Female
/
Humans
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Male
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Middle aged
Idioma:
En
Revista:
Blood
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Holanda