Genetic and enzymatic evidence for Lewis enzyme expression in Lewis-negative cancer patients.
Cancer Res
; 55(7): 1473-8, 1995 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-7882355
ABSTRACT
It has been observed that the frequency of individuals with Lewis-negative erythrocytes is significantly higher in cancer patients than in healthy controls. In this study, 20 of the 66 (30.3%) patients with various cancers were typed as Lewis negative from their erythrocytes, while the same frequency in healthy controls was 11.1%. These 20 patients were divided into three groups based on the presence of Lewis blood group antigens and alpha 1-->4-fucosyltransferase in their salivas group I, 6 patients who had both Lewis antigens and alpha 1-->4-fucosyltransferase activity; group II, 8 patients who had no Lewis antigens but possessed alpha 1-->4-fucosyltransferase activity; group III, 6 patients who had neither Lewis antigens nor alpha 1-->4-fucosyltransferase activity. The genotyping of Le genes by the PCR-RFLP methods, which have been developed and established by us recently, demonstrated that all 14 patients from groups I and II possess Le gene homozygously (Le/Le) or heterozygously (Le/le), whereas all 6 patients from group III were le/le homozygotes. Only the 6 patients from group III were identified as the genuine Lewis-negative individuals. The immunohistochemical staining of the colorectal tumors also showed that the Lewis antigens could be detected on the tumors from groups I and II but not from group III.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saliva
/
Neoplasias Esofágicas
/
Eritrócitos
/
Fucosiltransferases
/
Neoplasias Gastrointestinais
/
Antígenos do Grupo Sanguíneo de Lewis
Limite:
Humans
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Japão