Isolation of mouse complement component C7.
J Immunol Methods
; 176(2): 163-7, 1994 Dec 02.
Article
em En
| MEDLINE
| ID: mdl-7983377
ABSTRACT
Mouse complement component C7 was purified from serum by a sequential procedure of fractionation precipitation by ammonium sulfate, followed by DE-52 anion exchange chromatography. Protein G affinity column chromatography, Mono S cation exchange chromatography and Superdex 200 gel filtration. The final product contained a highly purified mouse C7 component showing a single band on SDS-PAGE at the apparent Mrs of 90 kDa and 100 kDa under non-reduced and reduced conditions respectively. The yield of C7, which was measured by the biological activity, was 7.0%
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Complemento C7
Limite:
Animals
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Japão