Improved detection of loss of heterozygosity at retinoblastoma gene locus in human breast carcinoma.
Pathol Int
; 44(1): 34-8, 1994 Jan.
Article
em En
| MEDLINE
| ID: mdl-8025647
ABSTRACT
Loss of heterozygosity (LOH) at the retinoblastoma (Rb) gene locus was investigated in 33 breast carcinomas by polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) analysis after tumor cell enrichment by cell sorting. The efficacy of cell sorting was evaluated by comparing the results of PCR-SSCP with and without cell sorting. Ten of 17 (59%) informative cases showed LOH at this locus by cell sorting combined with PCR-SSCP, although LOH was detectable in six (35%) cases without cell sorting. Flow cytometry and histologic examination revealed that this underestimation may occur when the tumor cell population is less than 50% in the specimens analyzed. It is concluded that LOH of the Rb gene occurs more frequently in human breast carcinoma than previously thought, and thus may contribute significantly to the development and/or progression of this tumor.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Genes do Retinoblastoma
/
Deleção Cromossômica
Tipo de estudo:
Diagnostic_studies
Limite:
Female
/
Humans
Idioma:
En
Revista:
Pathol Int
Assunto da revista:
PATOLOGIA
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Japão