Effect of controlled local acetylsalicylic acid release on in vitro platelet adhesion to vascular grafts.
J Biomater Appl
; 8(4): 361-84, 1994 Apr.
Article
em En
| MEDLINE
| ID: mdl-8064590
ABSTRACT
Thrombosis is the most serious acute problem for small diameter arterial bypass grafts. In this research, small diameter expanded polytetrafluoroethylene (e-PTFE) vascular grafts were coated with acetylsalicylic acid (ASA) loaded poly (d,l-lactide) (PLA) by a solvent casting method. The feasibility and efficacy of this approach were evaluated by ASA release studies and platelet adhesion tests. First, the ASA release kinetics were evaluated from the ASA/PLA coated vascular grafts in an in vitro steady flow loop model. ASA release was measured by a spectrophotometric technique. Finally, the efficacy of local ASA release to reduce in vitro canine platelet adhesion to grafts was determined with epifluorescent video microscopy and quantitative image analysis. The steady state release rates from the 5%, 10%, and 15% ASA/PLA coated grafts were 13.2 x 10(-5), 32.0 x 10(-5), and 41.5 x 10(-5) micrograms/cm2.sec, respectively. Platelet adhesion to 10% and 15% ASA/PLA coated grafts was reduced with respect to the control and 5% grafts for 10 days. Platelet adhesion to 5% ASA/PLA coated grafts was reduced with respect to controls at 2 and 10 days, but not initially.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Politetrafluoretileno
/
Prótese Vascular
/
Adesividade Plaquetária
/
Aspirina
/
Ácido Láctico
Limite:
Animals
Idioma:
En
Revista:
J Biomater Appl
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
1994
Tipo de documento:
Article