Immunoregulatory characteristics of the in vitro anti-ssDNA response.
Immunol Res
; 12(4): 349-57, 1993.
Article
em En
| MEDLINE
| ID: mdl-8151158
ABSTRACT
Continuous blockade of B-cell antigen receptors (BCRs) with Fab alpha sIg prevents the anti-ssDNA response of high, but not low, density B cells. Signaling via the BCRs, by prior exposure to crosslinking F(ab')2 alpha sIg, had no effect on the spontaneous anti-DNA response, but prevented a lipopolysaccharide-induced anti-DNA response. Pretreatment with intact alpha sIg, which provides exogenously derived Fc signals, reduced the response. An Fc-signal-blocking agent, F(ab')2 anti-IgG-Fc antibody, increased the number of anti-DNA antibody-forming cells produced in the absence of exogenous IgG anti-ssDNA antibody. Thus, activation is dependent on the availability of the BCRs, prior BCR crosslinking does not interfere with activation, and endogenous IgG anti-ssDNA antibody limits the activation of anti-ssDNA-specific B cells most of which are T-cell independent. These results indicate that the anti-ssDNA response is driven through the BCR.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA de Cadeia Simples
/
Receptores de Antígenos de Linfócitos B
/
Anticorpos Antinucleares
Limite:
Animals
Idioma:
En
Revista:
Immunol Res
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Canadá