Differential N-acetylation of alpha-MSH and beta-endorphin in the intermediate pituitary of the turtle, Pseudemys scripta.
Peptides
; 14(4): 849-55, 1993.
Article
em En
| MEDLINE
| ID: mdl-8234035
ABSTRACT
Steady-state analyses of the intermediate pituitary of the turtle, Pseudemys scripta, indicated that alpha-MSH-sized immunoreactive forms and beta-endorphin-sized immunoreactive forms are major end products of melanotropic cells. Three forms of alpha-MSH-related immunoreactivity were detected. The two major forms had the same reversed-phase HPLC properties as synthetic N,O-diacetyl-ACTH(1-13)-NH2 and N-acetyl-ACTH(1-13)-NH2. These forms accounted for 97% of the total alpha-MSH-related immunoreactivity detected. A minor peak of ACTH(1-13)-NH2 was also detected. Multiple forms of beta-endorphin-related immunoreactivity were detected, which varied in net positive charge (+1 to +5), apparent molecular weight (2.4 to 3.5 kDa), and degree of N-terminal acetylation. Although N-acetylated forms of beta-endorphin were detected in the turtle intermediate pituitary, the major forms of turtle beta-endorphin were nonacetylated. These features of the turtle intermediate pituitary POMC-specific N-acetylation mechanism are similar to, yet distinct from, the POMC N-acetylation mechanisms observed for mammals. These data suggest that POMC-specific N-acetylation mechanisms were present in reptiles prior to the divergence of the anapsid and synapsid lines.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Hipófise
/
Tartarugas
/
Alfa-MSH
/
Beta-Endorfina
Limite:
Animals
Idioma:
En
Revista:
Peptides
Ano de publicação:
1993
Tipo de documento:
Article