An optical hydroxyl radical sensor.
Biosens Bioelectron
; 8(6): 325-9, 1993.
Article
em En
| MEDLINE
| ID: mdl-8251135
ABSTRACT
A hydroxyl radical (.OH) fibre-optic sensor has been developed. An .OH radical-sensitive reagent phase (nitrophenol) was immobilized onto XAD-7 methacrylate beads. Subsequently the beads were attached to the distal end of a polymethylmethacrylate fibre optic. Nitrocatechol, generated from the attack of .OH radical on nitrophenol, exhibits a strong absorption band in the visible region of the electromagnetic spectrum (lambda max = 510 nm). Here, reflectance spectroscopy was employed to monitor the concomitant intensity decrease in the reflectance spectrum upon .OH radical attack. The sensor exhibited excellent stability and linearity of response to .OH generated by a Fenton reaction system (EDTA, Fe(II) and H2O2) with H2O2 over the concentration range of 3.6 x 10(-6)-8.0 x 10(-2) M.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Radical Hidroxila
/
Tecnologia de Fibra Óptica
Idioma:
En
Revista:
Biosens Bioelectron
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Reino Unido