Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate.
FEMS Microbiol Lett
; 110(1): 101-6, 1993 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-8319887
ABSTRACT
The recombinant plasmid pAL-A3 bears a (poly ManA) alginate lyase-encoding gene that originates from the marine bacterium ATCC 433367 (Brown et al., Appl. Environ. Microbiol. (1991) 57, 1870-1872). The alginate lyase produced by Escherichia coli TC4 harbouring pAL-A3 was purified to protein homogeneity and the corresponding gene sequenced, giving access to the first known primary structure of an alginate lyase. The 265-amino acid residue alginate lyase showed lytic activity on a Pseudomonas aeruginosa alginate isolated from a cystic fibrosis patient. Unexpectedly, the alginate lyase thus characterized differed from that isolated from the culture medium of the bacterium ATCC 433367 (Romeo and Preston, Biochemistry (1986) 25, 8385-8391).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeo-Liases
/
Pseudomonas aeruginosa
/
Proteínas de Bactérias
/
DNA Bacteriano
/
Alginatos
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
França