[Production of monoclonal antibody that recognizes glycogen and its application for immunohistochemistry].
Kokubyo Gakkai Zasshi
; 60(2): 264-87, 1993 Jun.
Article
em Ja
| MEDLINE
| ID: mdl-8345245
ABSTRACT
In this study, I made a monoclonal antibody, using the mandibular condylar cartilage as antigen. By light microscopic immunocytochemical observations, this antibody reacted with the chondrocytes both in the mandibular condyle and the costal cartilage, hepatocytes and skeletal muscle cells. By electron microscopic immunocytochemical observations, reaction products (gold particles) were seen on the glycogen particles in the chondrocytes. Further, by dot blotting assay, this antibody was found to react directly with the purified glycogen. Meanwhile, all these reactions disappeared after alpha-amylase digestion. These results indicate that this antibody specifically recognizes glycogen or glycogen-related sugar chains. Therefore, I think that this antibody is very much useful for detecting the glycogen instead of the PAS reaction, since PAS reaction is not a specific method to detect the glycogen.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glicogênio
/
Anticorpos Monoclonais
Limite:
Animals
Idioma:
Ja
Revista:
Kokubyo Gakkai Zasshi
Ano de publicação:
1993
Tipo de documento:
Article