Humoral stimulating activities in post-cyclophosphamide rat sera and their purified fractions.
Cell Prolif
; 26(1): 1-11, 1993 Jan.
Article
em En
| MEDLINE
| ID: mdl-8439585
ABSTRACT
Enhanced humoral, stimulating activities (HSAs) of post-cyclophosphamide (CY) sera and their purified fractions, acting on mitogen activated T-lymphocytes, were detected in Wistar rats after treatment by high single doses of the aplasia producing alkylating cytostatic drug CY. These activities, monitored in vitro, were partially purified from post-CY sera, collected 2, 4, and 7 days after treatment, yielding fractions with higher humoral stimulating activity. The preliminary purification included molecular cutting by Amicon-Diaflo filters (1-30 kDa pore size range) and gel-filtration on Sephadex G-50 and G-75. Results show that post-CY sera partially purified fraction (10-20 kDa), enhances the proliferation of hydrocortisone resistant (HCR) T-lymphocytes up to threefold under microculture conditions (P << 0.001); partially purified post-CY sera fraction (10-20 kDa) increases the proliferative response of T-cells in microcultures to the mitogenic 145-2C11 monoclonal antibody (mAb) up to tenfold (P << 0.001). These results show that the activity of stimulating factors is localized in the molecular weight range of 10-20 kDa.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Timidina
/
Proteínas Sanguíneas
/
Linfócitos T
/
Divisão Celular
/
Ciclofosfamida
Limite:
Animals
Idioma:
En
Revista:
Cell Prolif
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Iugoslávia