Selection of single-stranded deoxyribonucleic acid spermatozoa via the SpermPrep filtration column.
Fertil Steril
; 59(3): 690-2, 1993 Mar.
Article
em En
| MEDLINE
| ID: mdl-8458481
ABSTRACT
Eighteen semen samples were collected from 18 normospermic men. Two aliquots (1 mL) were prepared from each ejaculate, washed with Ham's F-10, and each washed sperm pellet was reconstituted in 2 mL volume of Ham's F-10 medium. Each aliquot one was stained using the AO-staining method. Each aliquot two was filtered via the SpermPrep II method, and the recovered spermatozoa were stained similarly. The proportion of single-stranded DNA (red) spermatozoa to double-stranded (green) spermatozoa was significantly higher in aliquot one than in the postfiltered sample (aliquot two), suggesting that the SpermPrep filtration procedure selectively entrapped the spermatozoa with abnormal DNA.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
/
DNA de Cadeia Simples
Limite:
Humans
/
Male
Idioma:
En
Revista:
Fertil Steril
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Japão