Detection of a prfA-independent promoter responsible for listeriolysin gene expression in mutant Listeria monocytogenes strains lacking the PrfA regulator.
Infect Immun
; 61(7): 3073-5, 1993 Jul.
Article
em En
| MEDLINE
| ID: mdl-8514414
ABSTRACT
Expression of listeriolysin, a major virulence factor of pathogenic Listeria monocytogenes, is positively regulated by the pleiotropic virulence regulator PrfA. In this study, we demonstrate that L. monocytogenes strains lacking the prfA regulator gene produce listeriolysin in small, albeit detectable, amounts when analyzed in a hemolysin assay and by immunoblots with listeriolysin-specific monoclonal antibodies. Transcriptional analysis revealed the existence of a PrfA-independent promoter that was responsible for the hemolytic activity expressed by these strains.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Toxinas Bacterianas
/
Transativadores
/
Genes Reguladores
/
Regiões Promotoras Genéticas
/
Genes Bacterianos
/
Proteínas de Choque Térmico
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Proteínas Hemolisinas
/
Listeria monocytogenes
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Infect Immun
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Alemanha