A gas-chromatographic assay method for busulfan with sensitivity for test dose therapeutic monitoring.
J Pharm Biomed Anal
; 13(9): 1073-8, 1995 Aug.
Article
em En
| MEDLINE
| ID: mdl-8573630
ABSTRACT
A gas-chromatographic assay method was developed and validated for determination of busulfan in human plasma for test dose therapeutic drug monitoring. Busulfan and the internal standard (1,6-bis-(methanesulfonyloxy)hexane) were extracted from plasma samples and derivatized with 2,3,5,6-tetrafluorothiophenol prior to gas chromatographic determination. The 63Ni electron-capture detector provided a limit of quantitation of 0.0100 micrograms ml-1 busulfan in plasma with a linear response over the concentration range 0.0100-0.400 micrograms ml-1. Extraction and derivatization yields were 85.3%-91.0% and greater than 95%, respectively. Assay specificity for busulfan in the presence of potential metabolites was demonstrated. Potentially co-administered drugs gave no response under the sample preparation and chromatographic conditions described for quantification of busulfan. The applicability of this assay to the individualization of busulfan therapy based on a 2 mg test dose is discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bussulfano
/
Cromatografia Gasosa
/
Antineoplásicos Alquilantes
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Canadá