A new model of transient cerebral ischemia in neonatal rats.
J Cereb Blood Flow Metab
; 16(2): 237-43, 1996 Mar.
Article
em En
| MEDLINE
| ID: mdl-8594055
ABSTRACT
A new model of transient cerebral ischemia in 10-day- old rats is described. Under microscopic guidance, the right external and internal carotid arteries were electrically coagulated. A solid 0.47 mm diameter nylon thread was inserted into the right common carotid artery toward the ascending aorta up to 10-12 mm from the upper edge of the sternomastoid muscle (preischemic rats). A 60-min cerebral ischemia was induced by clamping the left external and internal carotid arteries (ischemic rats), followed by 3-h recirculation. 31P magnetic resonance (MRS) spectroscopic studies revealed that severe intracellular acidosis occurred and ATP disappeared completely for a least the last 20 min of ischemia. Cerebral blood flow (CBF), measured by the hydrogen clearance technique, decreased to approximately 11% of the preischemic level in the frontal cortex soon after the induction of ischemia. On resuscitation, ATP recovered completely and the preischemic intracellular pH level was restored within 180 min. CBF has recovered to approximately 30% of the preischemic level at 5 min after resuscitation. The CBF recovery was not compete even at 180 min after resuscitation. With this model, the effects of pure ischemia without hypoxia on the neonatal brain and the process of recovery from transient ischemia can be studied.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carbono
/
Ataque Isquêmico Transitório
/
Modelos Animais de Doenças
/
Animais Recém-Nascidos
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
J Cereb Blood Flow Metab
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Japão