Effective 30-hour preservation of canine lungs with modified ET-Kyoto solution.
Ann Thorac Surg
; 61(4): 1099-105, 1996 Apr.
Article
em En
| MEDLINE
| ID: mdl-8607664
ABSTRACT
BACKGROUND:
With the aim of developing a preservation solution that can preserve donor lungs reliably for a long time, we prepared a modified ET-Kyoto solution by adding N-acetylcysteine, nitroglycerin, and dibutyryl adenosine 3', 5'-cyclic phosphate to the previously reported ET-Kyoto solution, which contains trehalose, gluconate, and hydroxyethyl starch. In this study, we examined the efficacy of modified ET-Kyoto solution in 30-hour lung preservation.METHODS:
Twenty five pairs of adult mongrel dogs were divided into four groups. Donor lungs were flushed with modified ET-Kyoto solution (n = 9), with ET-Kyoto solution (n = 6), with University of Wisconsin solution group (n = 6), or with ET-Kyoto solution plus the solvents of nitroglycerin (ethanol and propylene glycol) (n = 4), then stored at 4 degrees C for 30 hours. All animals were treated with prostaglandin E1. Left lungs were transplanted and reperfused for 6 hours.RESULTS:
With respect to arterial oxygen tension, peak inspiratory pressure, and wet-to-dry lung weight ratio, modified ET-Kyoto solution was significantly superior to ET-Kyoto solution. The modified ET-Kyoto solution was significantly superior to University of Wisconsin solution with respect to survival rate, arterial oxygen tension, and wet-to-dry lung weight ratio. Ultrastructural findings supported these results.CONCLUSIONS:
These results suggest that modified ET-Kyoto solution is superior to University of Wisconsin solution for 30-hour lung preservation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Preservação de Órgãos
/
Acetilcisteína
/
Trealose
/
Derivados de Hidroxietil Amido
/
Nitroglicerina
/
Bucladesina
/
Soluções para Preservação de Órgãos
/
Gluconatos
/
Pulmão
Limite:
Animals
Idioma:
En
Revista:
Ann Thorac Surg
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Japão