Formation of chimeric DNA primer extension products by template switching onto an annealed downstream oligonucleotide.
Proc Natl Acad Sci U S A
; 93(7): 2969-74, 1996 Apr 02.
Article
em En
| MEDLINE
| ID: mdl-8610152
ABSTRACT
Oligodeoxynucleotide sequences are described that anneal to a template downstream of a priming site. During polymerase-catalyzed extension of the primer, the extending primer shifts from the original template to a segment of the annealed oligonucleotide that acts as an alternative template. The resulting chimeric extended primer has one segment that is complementary to the template and a second segment that is complementary to the oligonucleotide. The influence of the sequence elements of the oligonucleotide and the reaction conditions on template switching have been explored. The sequence requirements for template switching are compared to those for transposon excision.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Primers do DNA
/
DNA Polimerase Dirigida por DNA
/
Conformação de Ácido Nucleico
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Estados Unidos