Increased expression of plasminogen activator inhibitor-1 in R. rickettsii-infected endothelial cells.
Thromb Haemost
; 75(4): 600-6, 1996 Apr.
Article
em En
| MEDLINE
| ID: mdl-8743186
ABSTRACT
Changes in PAI-1 expression in human umbilical vein endothelial cells (HUVEC) were studied following in vitro infection with Rickettsia rickettsii. A 1.8-fold increase in secreted PAI-1 activity occurred in infected versus control cultures (p = 0.03) at 24 h but not at earlier timepoints. A similar increase (1.4-fold) in secreted PAI-1 antigen (p < 0.005) was measured by ELISA. To determine whether this increase was due to increased synthesis of PAI-1, HUVEC were metabolically labeled with 35S-methionine concurrent with R. rickettsii infection. Such infection resulted in a 1.9-fold increase in labeled PAI-1 in the medium at 24 h (p = 0.036). Increase steady-state levels of PAI-1 mRNA were detected as early as 18 h by Northern blot analysis, peaking (5.5-fold) at approximately 24 h. These results indicate that PAI-1 production is increased in RR-infected endothelial cells, an effect that may contribute to the vascular occlusions noted in Rocky Mountain spotted fever.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Febre Maculosa das Montanhas Rochosas
/
Endotélio Vascular
/
Inibidor 1 de Ativador de Plasminogênio
Limite:
Humans
Idioma:
En
Revista:
Thromb Haemost
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Estados Unidos