Re-evaluation of the concept that high cell concentrations "protect" cells in vitro from ultrasonically induced lysis.
Ultrasound Med Biol
; 22(4): 497-514, 1996.
Article
em En
| MEDLINE
| ID: mdl-8795177
ABSTRACT
Diminution of apparent ultrasonic cell lysis in vitro with increasing cell concentration/volume fraction has often been observed. A substantial fraction of the cells may be lysed by ultrasound at low cell concentrations, but only a few percent are lysed when the concentration is a fraction of that of whole blood. This suggests that sonolysis of cells in vitro is suppressed by high cell concentrations, and, therefore, that sonolysis of cells in vivo is unlikely. We present the results of a retrospective analysis of experimental data and a theoretical analysis; these indicate that while relative sonolytic yield declines with increasing cell concentration, the "absolute" extent of ultrasound-induced lysis remains large. We find evidence that in vitro sonolysis of cells is limited at high cell densities by the number of available microbubbles and/or the number of cells each bubble may encounter and lyse prior to bubble "inactivation." Theory indicates the latter arises in consequence to the cell concentration-dependent formation of cell aggregates around pulsating bubbles.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Tumorais Cultivadas
/
Ultrassonografia
/
Eritrócitos
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Observational_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Ultrasound Med Biol
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Estados Unidos