Lipoxygenase activity in heart cells.
FEBS Lett
; 395(2-3): 148-52, 1996 Oct 21.
Article
em En
| MEDLINE
| ID: mdl-8898083
ABSTRACT
Arachidonic acid (AA) metabolism via the lipoxygenase (LOX) pathway in rat hearts and in cultured rat cardiomyocytes was investigated using 1-[14C]AA. LOX activity was detected in the microsomal fraction, in the high speed supernatant prepared from rat hearts and in rat cardiomyocyte supernatant. LOX products from all fractions comigrated in thin layer chromatography as 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-HETE. Enzyme linked immunosorbent assay for 12-HETE showed its formation by the microsomal fraction, the ammonium sulfate (AS) pellet, and by rat cardiomyocyte supernatant, while radioimmunoassay for 15-HETE showed its formation only by the AS pellet. The properties of LOX in each fraction are reported here.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lipoxigenase
/
Microssomos
/
Miocárdio
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Israel