Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells.
Brain Res
; 737(1-2): 351-5, 1996 Oct 21.
Article
em En
| MEDLINE
| ID: mdl-8930391
ABSTRACT
In digitonin-permeabilized adrenal chromaffin cells, Ca(2+)-induced catecholamine release can be resolved into at least two sequential steps:
a MgATP-dependent priming step and a MgATP-independent Ca(2+)-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca(2+)-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trifosfato de Adenosina
/
Células Cromafins
/
Proteínas de Membrana
/
Proteínas do Tecido Nervoso
Limite:
Animals
Idioma:
En
Revista:
Brain Res
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Japão