[Functional screening of p53 status in tumor cells using Saccharomyces cerevisiae].
Gan To Kagaku Ryoho
; 24(4): 466-70, 1997 Feb.
Article
em Ja
| MEDLINE
| ID: mdl-9063485
ABSTRACT
We have detected both germ-line and somatic p53 mutations in lymphocytes, cell lines and tumor tissues using a functional analysis of p53 tumor suppressor gene based on yeast transcription assay. Through our screening projects of the p53 gene, a number of missense p53 mutations were identified as loss-of-function mutations. This method, previously termed FASAY, is rapid, sensitive, less-expensive and can be automated for screening both somatic and germ-line p53 mutations.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Transcrição Gênica
/
Genes p53
/
Mutação
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
Ja
Revista:
Gan To Kagaku Ryoho
Ano de publicação:
1997
Tipo de documento:
Article