Stimulator of proliferation of spleen colony-forming cells in acute sterile inflammation.
Cell Prolif
; 26(6): 503-10, 1993 Nov.
Article
em En
| MEDLINE
| ID: mdl-9116117
ABSTRACT
The presence and activity of a spleen colony-forming cell (CFU-S) proliferation stimulator was investigated in rat bone marrow after induction of sterile inflammation. Wistar rats were treated intraperitoneally with two 15 ml injections of 3.5% polyvinylpyrrolidone (PVP) at 18 h intervals, and the presence of CFU-S proliferation stimulator determined in bone marrow 6, 20 and 24 h after the first injection. The marrow of these mice was used to condition medium which was then fractionated using Amicon Diaflo ultrafiltration membranes. The 30-50 kDa fraction, taken from 20h post-PVP-bone marrow extract, was found to induce cycling of d8-CFU-S in normal mouse bone marrow. This activity was not related to the presence of interleukins-1, -2 or -6 like activities in the material tested. The results demonstrate the existence of CFU-S proliferation stimulator in the bone marrow of rats with sterile inflammation (i.e. in an in vivo tissue response to non-specific cell stimulation), similar to that originally described as a macrophage product in mouse bone marrow after treatment with a variety of cytotoxic agents.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Baço
/
Medula Óssea
/
Células-Tronco Hematopoéticas
/
Substâncias de Crescimento
/
Inflamação
Limite:
Animals
Idioma:
En
Revista:
Cell Prolif
Ano de publicação:
1993
Tipo de documento:
Article