Does mammalian heart contain only the M2 muscarinic receptor subtype?
Life Sci
; 60(13-14): 1023-9, 1997.
Article
em En
| MEDLINE
| ID: mdl-9121343
ABSTRACT
Five muscarinic acetylcholine receptor (mAChR) subtypes, m1-m5, have been cloned and sequenced to date. The question as to which mAChR subtypes exist in mammalian heart has been studied extensively and is still under considerable debate. We used the reverse transcriptase-polymerase chain reaction to amplify mRNA from adult rat ventricular myocytes, and found that these cells express mRNA for m1 and m2 mAChRs. Immunocytochemical analysis confirmed that m1 and m2, but not m3, mAChR proteins are present on the surface of these cells. Finally, the functional significance of these receptors was examined. Administration of the m1 mAChR antagonist pirenzepine inhibited the stimulatory effect of the muscarinic agonist carbachol on Ca transients. These findings are consistent with the presence of at least two mAChR subtypes in mammalian heart, m1 and m2, and suggest that activation of m1 mAChRs is involved in the stimulatory effects of muscarinic agonists in mammalian heart.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores Muscarínicos
/
Miocárdio
Limite:
Animals
Idioma:
En
Revista:
Life Sci
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos