Differential mRNA expression in untreated and TNF-alpha elicited murine dendritic cells precursors.
Adv Exp Med Biol
; 417: 467-73, 1997.
Article
em En
| MEDLINE
| ID: mdl-9286405
ABSTRACT
We have compared the pattern of gene expression in long term cultured precursor dendritic cells (DC), either untreated (immature) or cultured for two days in the presence of recombinant murine (rm)-TNF alpha (mature). The hybridization signature of complex cDNA probes prepared from total RNA extracted from immature and mature DC were analyzed using a mouse thymic cDNA library, gridded on high density filters. For each clone spotted on the filters, we have measured using an imaging plate device the hybridization signals of the complex probe obtained from immature or mature DC. Comparative analysis of these values allowed us to identify differentially expressed gene products. Our goal is to identify a new set of genes induced or repressed during DC maturation elicited by rmTNF alpha treatment.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
/
RNA Mensageiro
/
Células-Tronco Hematopoéticas
/
Fator de Necrose Tumoral alfa
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Adv Exp Med Biol
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
França