Molecular cloning of a monoclonal anti-tumor antibody specific for the Tn antigen and expression of an active single-chain Fv fragment.
Hybridoma
; 16(4): 317-24, 1997 Aug.
Article
em En
| MEDLINE
| ID: mdl-9309422
ABSTRACT
We report here the first amino acid sequence of an anti-Tn monoclonal antibody raised against human breast cancer cells and show that a single chain Fv fragment of this IgM retains the Tn-binding specificity as defined by functional assays with asialo-OSM and membrane extracts from MCF-7 cells. Sequence comparisons and molecular modeling of 83D4 indicate that the antibody combining site displays a cavity-like feature primarily defined by the CDR H1 and H2 loops. This pocket could accommodate a single Tn molecule, thus, suggesting a structural explanation for the predominant expression of a particular VH gene segment in a group of antibodies that recognize tumor-associated antigens arising from an aberrant O-glycosylation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina M
/
Região Variável de Imunoglobulina
/
Fragmentos de Imunoglobulinas
/
Antígenos Glicosídicos Associados a Tumores
/
Cadeias kappa de Imunoglobulina
/
Anticorpos Monoclonais
/
Anticorpos Antineoplásicos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Hybridoma
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Uruguai