Transcriptional silencing by the mycobacteriophage L5 repressor.
EMBO J
; 16(19): 5914-21, 1997 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-9312049
ABSTRACT
The success of a temperate bacteriophage is dependent upon its ability to completely shut down expression of its lytic genes during lysogenic growth. Mycobacteriophage L5 accomplishes this by an atypical phage repressor, gp71, which binds to multiple asymmetric DNA sites. L5 gp71 regulates transcription initiation at an early lytic promoter, Pleft, but also affects downstream gene expression at 'stoperator' sites in the phage genome. The L5 genome is replete with stoperator sites located within short intergenic spaces in both the early and late lytic operons and oriented specifically with respect to transcription. Binding of gp71 to these sites results in a strong orientation-dependent polar effect on downstream gene expression and global silencing of prophage gene expression.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
Transcrição Gênica
/
Proteínas Virais
/
Regulação Viral da Expressão Gênica
/
Proteínas de Ligação a DNA
/
Micobacteriófagos
Idioma:
En
Revista:
EMBO J
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos