Affinity purification of a correctly folded fragment of synthetic HIV-1 mRNA using a HIV-1 Rev peptide-ligand.
Biomed Pept Proteins Nucleic Acids
; 2(2): 47-50, 1996.
Article
em En
| MEDLINE
| ID: mdl-9346826
ABSTRACT
Formation of a macromolecular complex between the RNA binding protein HIV-1 Rev and HIV-1 mRNA is an essential prerequisite for nuclear export and subsequent expression of HIV-1 mRNA. The arginine rich peptide TRQARRNRRRRWRARQR, corresponding to residues 34-50 of HIV-1 Rev, contains the mRNA binding motif. We prepared a thioether linked Rev34-50-cellulose conjugate to affinity purify a fragment of synthetic mRNA corresponding to the high affinity binding site for Rev. The correctly folded fraction of mRNA (27.5%) was isolated from a crude synthetic mixture.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
RNA Viral
/
Produtos do Gene rev
/
HIV-1
/
Conformação de Ácido Nucleico
Idioma:
En
Revista:
Biomed Pept Proteins Nucleic Acids
Assunto da revista:
BIOQUIMICA
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Austrália