Identification of partners of TIF34, a component of the yeast eIF3 complex, required for cell proliferation and translation initiation.
EMBO J
; 16(22): 6812-22, 1997 Nov 17.
Article
em En
| MEDLINE
| ID: mdl-9362495
ABSTRACT
Eukaryotic initiation factor-3 (eIF3) in the yeast Saccharomyces cerevisiae plays a central role in initiation of translation. The eIF3 complex contains at least eight different proteins, but, as yet, little is known about the function of the individual proteins. In this study we have characterized the role of TIF34 (eIF3-p39), a recently identified WD-40 domain-containing protein of 39 kDa, in the eIF3 complex. Using temperature-sensitive mutants of TIF34 we show that this protein is required for cell cycle progression and for mating and plays an essential role in initiation of protein synthesis. By two-hybrid screening we have identified two partners that directly associate with TIF34 PRT1, a previously characterized eIF3 subunit, and a novel protein of 33 kDa (eIF3-p33) which is part of the eIF3 complex and has an RNA binding domain. TIF34 and p33 interact with each other and overexpression of p33 complements the growth defect of a tif34-ts mutant. Our results provide support for both physical and functional interactions between three subunits, TIF34, PRT1 and p33, in the eIF3 complex.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Proteínas de Bactérias
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Fatores de Transcrição
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Biossíntese de Proteínas
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Fatores de Iniciação de Peptídeos
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Proteínas de Saccharomyces cerevisiae
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
EMBO J
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos