Nucleotide sequences of reptile calcitonins: their high homology to chicken calcitonin.
Zoolog Sci
; 14(5): 833-6, 1997 Oct.
Article
em En
| MEDLINE
| ID: mdl-9450396
ABSTRACT
The calcitonin genes of four species of reptiles (Reeve's turtle, rat snake, grass lizard, and spectacled caiman) were amplified from the genomic DNA, as well as from the mRNA of the ultimobranchial glands of the former three species, by the polymerase chain reaction (PCR) method, and were sequenced. Among several primer sets, only one primer set synthesized from the chicken calcitonin gene was compatible with those of the reptiles. The nucleotide sequences of the reptile calcitonin genes were highly homologous with that of chicken calcitonin (100% for turtle, 99% for caiman, 96% for lizard and 93% for snake). The products amplified from mRNA by the RT-PCR method matched completely those from genomic DNA in the turtle, snake and lizard.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Répteis
/
Calcitonina
/
Homologia de Sequência do Ácido Nucleico
/
Galinhas
/
Homologia de Sequência de Aminoácidos
Limite:
Animals
Idioma:
En
Revista:
Zoolog Sci
Assunto da revista:
BIOLOGIA
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Japão